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bv2 cells  (Elabscience Biotechnology)


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    Elabscience Biotechnology bv2 cells
    (A) MARK2 siRNA reduced MARK2 expression in <t>BV2</t> cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)
    Bv2 Cells, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bv2+cells+complete+medium/BV2+(DMEM)+Cell+Complete+Medium/bio_rxiv__2025__07__21__665902-41-0-2
    Average 93 stars, based on 22 article reviews
    bv2 cells - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "MARK2 in glial cells suppresses inflammatory responses and mitigates tau toxicity"

    Article Title: MARK2 in glial cells suppresses inflammatory responses and mitigates tau toxicity

    Journal: bioRxiv

    doi: 10.1101/2025.07.21.665902

    (A) MARK2 siRNA reduced MARK2 expression in BV2 cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)
    Figure Legend Snippet: (A) MARK2 siRNA reduced MARK2 expression in BV2 cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)

    Techniques Used: Expressing, Western Blot, Control, Quantitation Assay, Quantitative RT-PCR, Transfection

    (A-C) MARK2 knockdown enhances TLR-induced cytokine expression. BV2 cells were transfected with control or MARK2 siRNA and treated with Imiquimod (1μg/ml), 3p-hpRNA (100ng/ml), or DMXAA (10μg/ml) for 6 hours. Cells were subjected to qRT-PCR to analyze mRNA levels of IL-6 and IFNβ. N=3, Mean ± SD, N.S., p>0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test).
    Figure Legend Snippet: (A-C) MARK2 knockdown enhances TLR-induced cytokine expression. BV2 cells were transfected with control or MARK2 siRNA and treated with Imiquimod (1μg/ml), 3p-hpRNA (100ng/ml), or DMXAA (10μg/ml) for 6 hours. Cells were subjected to qRT-PCR to analyze mRNA levels of IL-6 and IFNβ. N=3, Mean ± SD, N.S., p>0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test).

    Techniques Used: Knockdown, Expressing, Transfection, Control, Quantitative RT-PCR

    Related Articles

    Expressing:

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo .
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. Te cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO2 37°C incubator.. Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. The cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO 2 37°C incubator.. The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.

    Western Blot:

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo .
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. Te cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO2 37°C incubator.. Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. The cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO 2 37°C incubator.. The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.

    Control:

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo .
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. Te cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO2 37°C incubator.. Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. The cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO 2 37°C incubator.. The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.

    Real-time Polymerase Chain Reaction:

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo .
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. Te cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO2 37°C incubator.. Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.Te cells were cotreated with diferent concentrations (10, 100, and 200 μM) of ITD and LPS (1 μg/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.

    Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo
    Article Snippet: BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).BV2 murine microglia cell line was purchased from Elabscience Biotechnology Inc (Elabscience, USA, TX, No. CRL-2266).. The cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO 2 37°C incubator.. The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.The cells were cotreated with different concentrations (10, 100, and 200 μ M) of ITD and LPS (1 μ g/ml) for 24 hr and harvested the cells for the western blot and qPCR analysis.



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    bv2 cells  (Elabscience Biotechnology)
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    (A) MARK2 siRNA reduced MARK2 expression in <t>BV2</t> cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)
    Bv2 Cells, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bv2+cells+complete+medium/BV2+(DMEM)+Cell+Complete+Medium/bio_rxiv__2025__07__21__665902-41-0-2
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    (A) MARK2 siRNA reduced MARK2 expression in <t>BV2</t> cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)
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    (A) MARK2 siRNA reduced MARK2 expression in <t>BV2</t> cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)
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    Image Search Results


    (A) MARK2 siRNA reduced MARK2 expression in BV2 cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)

    Journal: bioRxiv

    Article Title: MARK2 in glial cells suppresses inflammatory responses and mitigates tau toxicity

    doi: 10.1101/2025.07.21.665902

    Figure Lengend Snippet: (A) MARK2 siRNA reduced MARK2 expression in BV2 cells. Western blot of BV2 cells treated with control siRNA or MARK2 siRNA. Actin was used as a loading control. Representative blots and quantitation are shown. N=3, mean ± _SD, *, p < 0.05 (Student’s t-test) (B) mRNA expression of IL-6, IFNβ, IL-1β, TNFα, IL-10, IFNα was analyzed via qRT-PCR. BV2 cells were transfected with control siRNA or MARK2 siRNA and treated with LPS (1ng/ml) for 6 hours. N=3, Mean ± SD, N.S., p>0.05, *, p<0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test)

    Article Snippet: BV2 cells (Elabscience (Houston, TX)) were cultured in MEM (11095-080, Thermo Fisher Scientific) medium at 5% CO 2 and 37°C.

    Techniques: Expressing, Western Blot, Control, Quantitation Assay, Quantitative RT-PCR, Transfection

    (A-C) MARK2 knockdown enhances TLR-induced cytokine expression. BV2 cells were transfected with control or MARK2 siRNA and treated with Imiquimod (1μg/ml), 3p-hpRNA (100ng/ml), or DMXAA (10μg/ml) for 6 hours. Cells were subjected to qRT-PCR to analyze mRNA levels of IL-6 and IFNβ. N=3, Mean ± SD, N.S., p>0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test).

    Journal: bioRxiv

    Article Title: MARK2 in glial cells suppresses inflammatory responses and mitigates tau toxicity

    doi: 10.1101/2025.07.21.665902

    Figure Lengend Snippet: (A-C) MARK2 knockdown enhances TLR-induced cytokine expression. BV2 cells were transfected with control or MARK2 siRNA and treated with Imiquimod (1μg/ml), 3p-hpRNA (100ng/ml), or DMXAA (10μg/ml) for 6 hours. Cells were subjected to qRT-PCR to analyze mRNA levels of IL-6 and IFNβ. N=3, Mean ± SD, N.S., p>0.05, **, p<0.01, ***, p < 0.001 (One-way ANOVA followed by Tukey HSD test).

    Article Snippet: BV2 cells (Elabscience (Houston, TX)) were cultured in MEM (11095-080, Thermo Fisher Scientific) medium at 5% CO 2 and 37°C.

    Techniques: Knockdown, Expressing, Transfection, Control, Quantitative RT-PCR